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Melanophilin (mouse) Antibody

Goat polyclonal antibody to Mlph

Product Specifications

Product Name Alternative

Anti 2210418F23Rik antibody, anti melanophilin antibody, anti Mlph antibody, anti ln antibody, anti l1Rk3 antibody, anti Slac-2a antibody, anti D1Wsu84e antibody, anti l (1) -3Rk antibody, anti 5031433I09Rik antibody, anti leaden antibody, anti AW228792 antibody, anti OTTMUSP00000025451 antibody

Reactivity

Mouse

Target

Melanophilin (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ARHIFAKPVMAQQP

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5-2ug/ml

Molecular Weight

65

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/ml. Please note that this product may require overnight incubation of primary and high protein loads.Experiment Notes: Immunofluorescence: successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. Immunofluorescence: see also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283 (34) :23209-16. Epub 2008 Jun 17. (PMID:18559336) . GST-pull down assay: see publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283 (34) :23209-16. Epub 2008 Jun 17. (PMID:18559336) .

Prediction Reactivity

Rat

Tested Applications

ELISA, ICC, IF, Pull-down Assay, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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