Melanophilin (mouse) Antibody
Goat polyclonal antibody to Mlph
Product Specifications
Product Name Alternative
Anti 2210418F23Rik antibody, anti melanophilin antibody, anti Mlph antibody, anti ln antibody, anti l1Rk3 antibody, anti Slac-2a antibody, anti D1Wsu84e antibody, anti l (1) -3Rk antibody, anti 5031433I09Rik antibody, anti leaden antibody, anti AW228792 antibody, anti OTTMUSP00000025451 antibody
Reactivity
Mouse
Target
Melanophilin (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ARHIFAKPVMAQQP
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5-2ug/ml
Molecular Weight
65
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/ml. Please note that this product may require overnight incubation of primary and high protein loads.Experiment Notes: Immunofluorescence: successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. Immunofluorescence: see also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283 (34) :23209-16. Epub 2008 Jun 17. (PMID:18559336) . GST-pull down assay: see publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283 (34) :23209-16. Epub 2008 Jun 17. (PMID:18559336) .
Prediction Reactivity
Rat
Tested Applications
ELISA, ICC, IF, Pull-down Assay, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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