Human Granzyme A (GZMA) ELISA Kit

CTLA3 ELISA Kit, HFSP ELISA Kit, HF ELISA Kit, H factor ELISA Kit, Granzyme 1 ELISA Kit, Fragmentin-1 ELISA Kit, Hanukkah factor ELISA Kit, Cytotoxic T-Lymphocyte-Associated Serine Esterase 3 ELISA Kit, CTL tryptase ELISA Kit, Cytotoxic T-lymphocyte proteinase 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMA in the samples is then determined by comparing the OD of the samples to the standard curve