Human Mitogen Activated Protein Kinase 12 (MAPK12) ELISA Kit

ERK6 ELISA Kit, PRKM12 ELISA Kit, p38 Gamma ELISA Kit, SAPK3 ELISA Kit, Stress-activated protein kinase 3 ELISA Kit, Extracellular signal-regulated kinase 6 ELISA Kit, Mitogen-activated protein kinase p38 gamma ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK12 in the samples is then determined by comparing the OD of the samples to the standard curve