Human Signal Regulatory Protein Alpha (SIRPa) ELISA Kit

CD172a ELISA Kit, SIRP-A ELISA Kit, P84 ELISA Kit, BIT ELISA Kit, MFR ELISA Kit, MYD1 ELISA Kit, PTPNS1 ELISA Kit, SHPS1 ELISA Kit, SIRP ELISA Kit, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 ELISA Kit, Macrophage fusion receptor ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRPa in the samples is then determined by comparing the OD of the samples to the standard curve