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Human Filamin A Alpha (FLNa) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha (FLNa) .

Product Specifications

Product Name Alternative

ABP280 ELISA Kit, FLN-A ELISA Kit, ABPX ELISA Kit, FLN ELISA Kit, FLN1 ELISA Kit, FMD ELISA Kit, MNS ELISA Kit, NHBP ELISA Kit, OPD ELISA Kit, OPD1 ELISA Kit, OPD2 ELISA Kit, Actin Binding Protein 280 ELISA Kit, Endothelial actin-binding protein ELISA Kit, Filamin-1 ELISA Kit, Non-muscle filamin ELISA Kit

UniProt

P21333

Reactivity

Human

Field of Research

Musculoskeletal & Connective Tissue Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.62 ng/mL

Notes

For research use only.

Applications Notes

Standard: 120 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLNa in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

1.88-120 ng/mL

Available Sizes

Frequently Asked Questions

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