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Mouse Ataxia Telangiectasia Mutated (ATM) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxia Telangiectasia Mutated (ATM) .

Product Specifications

Product Name Alternative

AT1 ELISA Kit, ATD ELISA Kit, ATA ELISA Kit, ATC ELISA Kit, ATDC ELISA Kit, ATE ELISA Kit, TEL1 ELISA Kit, TELO1 ELISA Kit, Serine-protein kinase ATM ELISA Kit

UniProt

Q62388

Reactivity

Mouse

Field of Research

Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Sensitivity

6.2 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATM in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

Available Sizes

Frequently Asked Questions

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