Human Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit

CD363 ELISA Kit, EDG1 ELISA Kit, S1-PR1 ELISA Kit, S1P1 ELISA Kit, ECGF1 ELISA Kit, CHEDG1 ELISA Kit, Endothelial differentiation G-protein coupled receptor 1 ELISA Kit, Sphingolipid G-Protein-Coupled Receptor 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S1PR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S1PR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S1PR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S1PR1 in the samples is then determined by comparing the OD of the samples to the standard curve