Rat L1-Cell Adhesion Molecule (L1CAM) ELISA Kit

CD171 ELISA Kit, L1-CAM ELISA Kit, CAML1 ELISA Kit, HSAS ELISA Kit, HSAS1 ELISA Kit, MASA ELISA Kit, MIC5 ELISA Kit, N-CAML1 ELISA Kit, S10 ELISA Kit, SPG1 ELISA Kit, Antigen Identified By Monoclonal Antibody R1 ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat L1CAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat L1CAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat L1CAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat L1CAM in the samples is then determined by comparing the OD of the samples to the standard curve