Human Thyroid Transcription Factor 1 (TITF1) ELISA Kit

TTF1 ELISA Kit, NKX2-1 ELISA Kit, BCH ELISA Kit, BHC ELISA Kit, NK-2 ELISA Kit, NKX2.1 ELISA Kit, NKX2A ELISA Kit, TEBP ELISA Kit, NK2 Homeobox 1 ELISA Kit, Benign Chorea ELISA Kit, Thyroid nuclear factor 1 ELISA Kit, Thyroid-specific enhancer-binding protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TITF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TITF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TITF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TITF1 in the samples is then determined by comparing the OD of the samples to the standard curve