Flotillin 1 Antibody
Goat polyclonal to Flotillin 1
Product Specifications
Product Name Alternative
Anti FLOT1 antibody, anti flotillin 1 antibody, anti reggie-2 antibody, anti integral membrane component of caveolae antibody, anti DADB-118P11.2 antibody
Reactivity
Human
Target
Flotillin 1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SISQVNHKPLRTA
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1-0.3 μg/ml
Molecular Weight
47.4; 42.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell line H460 (calculated MW of 47.4kDa according to NP_005794.1) . In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 μg/ml.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10211
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