Human Inhibitor of Kappa-Light Polypeptide Gene Enhancer in B-Cells Kinase Beta (IkBKb) ELISA Kit

IKK2 ELISA Kit, NFKBIKB ELISA Kit, IKK-beta ELISA Kit, IKKB ELISA Kit, I-kappa-B kinase 2 ELISA Kit, Nuclear factor NF-kappa-B inhibitor kinase beta ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBKb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBKb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBKb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBKb in the samples is then determined by comparing the OD of the samples to the standard curve