Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit

C-K-RAS ELISA Kit, K-RAS2A ELISA Kit, K-RAS2B ELISA Kit, K-RAS4A ELISA Kit, K-RAS4B ELISA Kit, KI-RAS ELISA Kit, KRAS1 ELISA Kit, KRAS2 ELISA Kit, NS3 ELISA Kit, RASK2 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRAS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRAS in the samples is then determined by comparing the OD of the samples to the standard curve