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Human Telomeric Repeat Binding Factor 1 (TERF1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 1 (TERF1) .

Product Specifications

Product Name Alternative

TRF ELISA Kit, PIN2 ELISA Kit, TRBF1 ELISA Kit, TRF1 ELISA Kit, hTRF1-AS ELISA Kit, t-TRF1 ELISA Kit, NIMA-interacting protein 2 ELISA Kit, TTAGGG repeat-binding factor 1 ELISA Kit, Telomeric protein Pin2/TRF1 ELISA Kit

UniProt

P54274

Reactivity

Human

Field of Research

Stem Cell & Developmental Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.058 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TERF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TERF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TERF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TERF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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