Human Protein Disulfide Isomerase A4 (PDIA4) ELISA Kit

PDI-A4 ELISA Kit, ERP70 ELISA Kit, ERP72 ELISA Kit, Protein Disulfide Isomerase-Associated 4 ELISA Kit, Endoplasmic reticulum resident protein 70 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA4 in the samples is then determined by comparing the OD of the samples to the standard curve