Rat S100 Calcium Binding Protein A10 (S100A10) ELISA Kit

ANX2L ELISA Kit, ANX2LG ELISA Kit, CAL1L ELISA Kit, CLP11 ELISA Kit, GP11 ELISA Kit, P11 ELISA Kit, P10 ELISA Kit, Calpactin-1 light chain ELISA Kit, Cellular ligand of annexin II ELISA Kit, Annexin II Ligand, Calpactin I, Light Polypeptide ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A10 in the samples is then determined by comparing the OD of the samples to the standard curve