Rat S100 Calcium Binding Protein A6 (S100A6) ELISA Kit

S100-A6 ELISA Kit, 2A9 ELISA Kit, 5B10 ELISA Kit, CABP ELISA Kit, CACY ELISA Kit, PRA ELISA Kit, MLN 4 ELISA Kit, Calcyclin ELISA Kit, Growth factor-inducible protein 2A9 ELISA Kit, Prolactin receptor-associated protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A6 in the samples is then determined by comparing the OD of the samples to the standard curve