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Human Karyopherin Alpha 2 (KPNa2) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2) .

Product Specifications

Product Name Alternative

IPOA1 ELISA Kit, QIP2 ELISA Kit, RCH1 ELISA Kit, SRP1alpha ELISA Kit, RAG Cohort 1 ELISA Kit, Importin Alpha 1 ELISA Kit, RAG cohort protein 1 ELISA Kit, SRP1-alpha ELISA Kit

UniProt

P52292

Reactivity

Human

Field of Research

Cell Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

50 pg/mL

Notes

For research use only.

Applications Notes

Standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KPNa2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KPNa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KPNa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KPNa2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

125-8000 pg/mL

Available Sizes

Frequently Asked Questions

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