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Human Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) .

Product Specifications

Product Name Alternative

DDAH ELISA Kit, G6A ELISA Kit, NG30 ELISA Kit, N (G), N (G) -dimethylarginine dimethylaminohydrolase 2 ELISA Kit, Dimethylargininase-2 ELISA Kit, Protein G6a ELISA Kit, S-phase protein ELISA Kit

UniProt

O95865

Reactivity

Human

Field of Research

Protein Biochemistry

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.112 ng/mL

Notes

For research use only.

Applications Notes

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDAH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDAH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDAH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDAH2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.32-20 ng/mL

Available Sizes

Frequently Asked Questions

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