Human Keratin 6A (KRT6A) ELISA Kit

K6A ELISA Kit, KRT6C ELISA Kit, KRT6D ELISA Kit, CK6C ELISA Kit, K6C ELISA Kit, CK6D ELISA Kit, K6D ELISA Kit, Keratin 6C ELISA Kit, Keratin 6D ELISA Kit, Keratin, type II cytoskeletal 6A ELISA Kit, Type-II keratin Kb6 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT6A in the samples is then determined by comparing the OD of the samples to the standard curve