Human Plectin (PLEC) ELISA Kit

HD1 ELISA Kit, PCN ELISA Kit, EBS1 ELISA Kit, EBSO ELISA Kit, PLEC1b ELISA Kit, PLTN ELISA Kit, Hemidesmosomal protein 1 ELISA Kit, Intermediate Filament Binding Protein 500kDa ELISA Kit, Epidermolysis Bullosa Simplex 1 (Ogna) ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLEC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLEC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLEC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLEC in the samples is then determined by comparing the OD of the samples to the standard curve