Human Flotillin 2 (FLOT2) ELISA Kit

ECS1 ELISA Kit, ESA ELISA Kit, ESA1 ELISA Kit, M17S1 ELISA Kit, Epidermal Surface Antigen 1 ELISA Kit, Membrane Component, Chromosome 17, Surface Marker 1 ELISA Kit, 35kD Protein Identified By Monoclonal Antibody ECS-1 ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLOT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLOT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLOT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLOT2 in the samples is then determined by comparing the OD of the samples to the standard curve