Human Keratin 7 (KRT7) ELISA Kit

CK7 ELISA Kit, K2C7 ELISA Kit, K7 ELISA Kit, SCL ELISA Kit, Cytokeratin 7 ELISA Kit, Sarcolectin ELISA Kit, Keratin,55K Type II Cytoskeletal ELISA Kit, Keratin, type II cytoskeletal 7 ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT7 in the samples is then determined by comparing the OD of the samples to the standard curve