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Mouse Pleiotrophin (PTN) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN) .

Product Specifications

Product Name Alternative

HARP ELISA Kit, HBGF8 ELISA Kit, HBNF ELISA Kit, NEGF1 ELISA Kit, Heparin Binding Growth Factor 8 ELISA Kit, Neurite Growth-Promoting Factor 1 ELISA Kit, Heparin Affin Regulatory Peptide ELISA Kit, Heparin Binding Growth Associated Molecule ELISA Kit

UniProt

P63089

Reactivity

Mouse

Field of Research

Cancer Biology, Immunology & Inflammation, Neuroscience

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Sensitivity

3.1 pg/mL

Notes

For research use only.

Applications Notes

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTN in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

7.82-500 pg/mL

Available Sizes

Frequently Asked Questions

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