Human Neudesin Neurotrophic Factor (NENF) ELISA Kit

CIR2 ELISA Kit, SCIRP10 ELISA Kit, NDNF ELISA Kit, SPUF ELISA Kit, Neudesin ELISA Kit, Cell immortalization-related protein 2 ELISA Kit, Neuron-derived neurotrophic factor ELISA Kit, Secreted protein of unknown function ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NENF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NENF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NENF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NENF in the samples is then determined by comparing the OD of the samples to the standard curve