Human Quinoid Dihydropteridine Reductase (QDPR) ELISA Kit

DHPR ELISA Kit, PKU2 ELISA Kit, SDR33C1 ELISA Kit, HDHPR ELISA Kit, 6,7-Dihydropteridine Reductase ELISA Kit, Short Chain Dehydrogenase/Reductase Family 33C, Member 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human QDPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human QDPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human QDPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human QDPR in the samples is then determined by comparing the OD of the samples to the standard curve