Rat Midkine (MK) ELISA Kit

MDK ELISA Kit, ARAP ELISA Kit, NEGF2 ELISA Kit, Neurite Growth-Promoting Factor 2 ELISA Kit, Amphiregulin-associated protein ELISA Kit, Midgestation and kidney protein ELISA Kit, Neurite outgrowth-promoting factor 2 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MK in the samples is then determined by comparing the OD of the samples to the standard curve