Rat Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit

LAP1 ELISA Kit, HSC54 ELISA Kit, HSC70 ELISA Kit, HSC71 ELISA Kit, HSP71 ELISA Kit, HSPA10 ELISA Kit, NIP71 ELISA Kit, HSP73 ELISA Kit, Heat shock cognate 71 kDa protein ELISA Kit, Lipopolysaccharide-associated protein 1 ELISA Kit, LPS-associated protein 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPA8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPA8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPA8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPA8 in the samples is then determined by comparing the OD of the samples to the standard curve