Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit

CL100 ELISA Kit, HVH1 ELISA Kit, MKP1 ELISA Kit, PTPN10 ELISA Kit, Mitogen-activated protein kinase phosphatase 1 ELISA Kit, Protein-tyrosine phosphatase CL100 ELISA Kit, Dual specificity protein phosphatase hVH1 ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DUSP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DUSP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DUSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DUSP1 in the samples is then determined by comparing the OD of the samples to the standard curve