Mouse Runt Related Transcription Factor 2 (RUNX2) ELISA Kit

AML3 ELISA Kit, CBFA1 ELISA Kit, CCD1 ELISA Kit, OSF2 ELISA Kit, PEBP2A ELISA Kit, Acute myeloid leukemia 3 ELISA Kit, Core-binding factor alpha-1 ELISA Kit, Osteoblast-specific transcription factor 2 ELISA Kit, Polyomavirus enhancer-binding protein 2 A ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RUNX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RUNX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RUNX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RUNX2 in the samples is then determined by comparing the OD of the samples to the standard curve