Human Ribonuclease III, Nuclear (RNASEN) ELISA Kit

RnaseIII ELISA Kit, RNASE3L ELISA Kit, Rnase-III ELISA Kit, Etohi2 ELISA Kit, DROSHA ELISA Kit, RN3 ELISA Kit, RNASE3-L ELISA Kit, Ribonuclease Type III ELISA Kit, Double-Stranded RNA-Specific Endoribonuclease ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RNASEN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RNASEN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RNASEN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RNASEN in the samples is then determined by comparing the OD of the samples to the standard curve