Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

DCDC3A ELISA Kit, DCLK ELISA Kit, DCAMKL1 ELISA Kit, Doublecortin And CaM Kinase-Like 1 ELISA Kit, Doublecortin domain-containing protein 3A ELISA Kit, Doublecortin-like and CAM kinase-like 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DCLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DCLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DCLK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DCLK1 in the samples is then determined by comparing the OD of the samples to the standard curve