Human Collagen Type XV (COL15) ELISA Kit

COL15A1 ELISA Kit, COL15-A1 ELISA Kit, Collagen Alpha-1 (XV) chain ELISA Kit, Restin ELISA Kit, Endostatin-XV ELISA Kit, Related to endostatin ELISA Kit, ELISA Kit

Standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL15 in the samples is then determined by comparing the OD of the samples to the standard curve