Human Poly ADP Ribose Polymerase 4 (PARP4) ELISA Kit

ADPRTL1 ELISA Kit, ARTD4 ELISA Kit, PARPL ELISA Kit, PH5P ELISA Kit, VAULT3 ELISA Kit, VPARP ELISA Kit, P193 ELISA Kit, VWA5C ELISA Kit, von Willebrand Factor A Domain Containing 5C ELISA Kit, ADP-ribosyltransferase diphtheria toxin-like 4 ELISA Kit, Vault polymerase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PARP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PARP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PARP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PARP4 in the samples is then determined by comparing the OD of the samples to the standard curve