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Human Peroxiredoxin 1 (PRDX1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 1 (PRDX1) .

Product Specifications

Product Name Alternative

MSP23 ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, TDPX2 ELISA Kit, Natural killer cell-enhancing factor A ELISA Kit, Proliferation-associated ELISA Kit, Thioredoxin peroxidase 2 ELISA Kit, Thioredoxin-dependent peroxide reductase 2 ELISA Kit

UniProt

Q06830

Reactivity

Human

Field of Research

Protein Biochemistry

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.06 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRDX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRDX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRDX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRDX1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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