Human Regenerating Islet Derived Protein 1 Beta (REG1b) ELISA Kit

PSPS2 ELISA Kit, REGH ELISA Kit, REGI-BETA ELISA Kit, REGL ELISA Kit, Pancreatic Stone Protein ELISA Kit, Pancreatic Thread Protein ELISA Kit, Lithostathine 1 Beta ELISA Kit, Secretory Pancreatic Stone Protein 2 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REG1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REG1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REG1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REG1b in the samples is then determined by comparing the OD of the samples to the standard curve