Human Inhibitor of Apoptosis Protein, X-Linked (XIAP) ELISA Kit

BIRC4 ELISA Kit, API3 ELISA Kit, ILP1 ELISA Kit, MIHA ELISA Kit, XLP2 ELISA Kit, Baculoviral IAP Repeat-Containing 4 ELISA Kit, E3 ubiquitin-protein ligase XIAP ELISA Kit, IAP-like ELISA Kit, Inhibitor of apoptosis protein 3 ELISA Kit, X-linked inhibitor of apoptosis protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XIAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XIAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XIAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XIAP in the samples is then determined by comparing the OD of the samples to the standard curve