Mouse Breast and Kidney Expressed Chemokine (BRAK) ELISA Kit

CXCL14 ELISA Kit, SCYB14 ELISA Kit, BRAK ELISA Kit, NJAC ELISA Kit, Bolekine ELISA Kit, Kec ELISA Kit, MIP2-G ELISA Kit, MIP2g ELISA Kit, BMAC ELISA Kit, KS1 ELISA Kit, Chemokine (C-X-C-Motif) ligand 14 ELISA Kit, Small Inducible Cytokine Subfamily B (Cys-X-Cys) Member 14 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BRAK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BRAK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BRAK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BRAK in the samples is then determined by comparing the OD of the samples to the standard curve