Human FK506 Binding Protein 5 (FKBP5) ELISA Kit

P54 ELISA Kit, FKBP51 ELISA Kit, FKBP54 ELISA Kit, Ptg-10 ELISA Kit, Rotamase ELISA Kit, 51 kDa FK506-binding protein ELISA Kit, 54 kDa progesterone receptor-associated ELISA Kit, Androgen-regulated protein 6 ELISA Kit, HSP90-binding immunophilin ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FKBP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FKBP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FKBP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FKBP5 in the samples is then determined by comparing the OD of the samples to the standard curve