Human N-Methylpurine DNA Glycosylase (MPG) ELISA Kit

AAG ELISA Kit, APNG ELISA Kit, ADPG ELISA Kit, CRA36.1 ELISA Kit, MDG ELISA Kit, Mid1 ELISA Kit, PIG11 ELISA Kit, PIG16 ELISA Kit, Anpg ELISA Kit, DNA-3-Methyladenine Glycosylase ELISA Kit, Alkyladenine DNA Glycosylase ELISA Kit, 3-alkyladenine DNA glycosylase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPG in the samples is then determined by comparing the OD of the samples to the standard curve