Human Collagen Type VII (COL7) ELISA Kit

COL7-A1 ELISA Kit, COL7A1 ELISA Kit, EBD1 ELISA Kit, EBDCT ELISA Kit, EBR1 ELISA Kit, Long-chain collagen ELISA Kit, Epidermolysis Bullosa, Dystrophic, Dominant And Recessive ELISA Kit, Collagen Alpha-1 (VII) chain ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL7 in the samples is then determined by comparing the OD of the samples to the standard curve