Rat A Disintegrin and Metalloprotease 10 (ADAM10) ELISA Kit

CD156-C ELISA Kit, CD156c ELISA Kit, MADM ELISA Kit, Kuz ELISA Kit, CDw156 ELISA Kit, Kuzbanian protein homolog ELISA Kit, Mammalian disintegrin-metalloprotease ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADAM10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADAM10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADAM10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADAM10 in the samples is then determined by comparing the OD of the samples to the standard curve