Human G Protein Coupled Receptor 37 (GPR37) ELISA Kit

EDNRBL ELISA Kit, PAELR ELISA Kit, hET (B) R-LP ELISA Kit, Endothelin Receptor Type B-Like ELISA Kit, Parkin-associated endothelin receptor-like receptor ELISA Kit, Prosaposin receptor GPR37 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPR37 in the samples is then determined by comparing the OD of the samples to the standard curve