Human Myocilin (MYOC) ELISA Kit

GLC1A ELISA Kit, GPOA ELISA Kit, JOAG ELISA Kit, JOAG1 ELISA Kit, TIGR ELISA Kit, Trabecular Meshwork Inducible Glucocorticoid Response ELISA Kit, Myocilin 55 kDa subunit ELISA Kit

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYOC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYOC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYOC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYOC in the samples is then determined by comparing the OD of the samples to the standard curve