Rabbit Enolase, Neuron Specific (NSE) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase, Neuron Specific (NSE) .
Product Specifications
Product Name Alternative
ENO2 ELISA Kit, Enolase 2 ELISA Kit, Gamma Enolase ELISA Kit, 2-phospho-D-glycerate hydro-lyase ELISA Kit, Neural enolase ELISA Kit
Reactivity
Rabbit
Field of Research
Neuroscience; Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.28 ng/mL
Notes
For research use only.
Applications Notes
Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NSE in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.63-40 ng/mL
Available Sizes
Frequently Asked Questions
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