Rat Endoglin (ENG) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoglin (ENG) .

Product Specifications

Product Name Alternative

CD105 ELISA Kit, END ELISA Kit, HHT1 ELISA Kit, ORW ELISA Kit, ORW1 ELISA Kit, Osler-Rendu-Weber Syndrome 1 ELISA Kit

Reactivity

Rat

Field of Research

Cardiovascular Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Sensitivity

0.34 ng/mL

Notes

For research use only.

Applications Notes

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ENG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ENG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ENG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ENG in the samples is then determined by comparing the OD of the samples to the standard curve