Rat Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit

CD253 ELISA Kit, TNFSF10 ELISA Kit, APO2L ELISA Kit, Apo2-L ELISA Kit, TL2 ELISA Kit, TRAIL ELISA Kit, Tumor Necrosis Factor Ligand Superfamily Member 10 ELISA Kit, Apo-2 Ligand ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRAIL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRAIL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRAIL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRAIL in the samples is then determined by comparing the OD of the samples to the standard curve