Human Fatty Acid Synthase (FASN) ELISA Kit

FAS ELISA Kit, OA519 ELISA Kit, SDR27X1 ELISA Kit, Short Chain Dehydrogenase/Reductase Family 27X, Member 1 ELISA Kit, S-acetyltransferase ELISA Kit, 3-oxoacyl synthase ELISA Kit, Oleoyl hydrolase ELISA Kit, Enoyl-acyl-carrier-protein reductase ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FASN in the samples is then determined by comparing the OD of the samples to the standard curve