Human Chemokine C-C-Motif Ligand 14 (CCL14) ELISA Kit

SCYA14 ELISA Kit, HCC-1 ELISA Kit, HCC-3 ELISA Kit, NCC-2 ELISA Kit, SCYL2 ELISA Kit, CKb1 ELISA Kit, MCIF ELISA Kit, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 14 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCL14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCL14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCL14 in the samples is then determined by comparing the OD of the samples to the standard curve