Mouse Galectin 8 (GAL8) ELISA Kit

LGALS8 ELISA Kit, PCTA1 ELISA Kit, Po66-CBP ELISA Kit, Prostate Carcinoma Tumor Antigen 1 ELISA Kit, Lectin, Galactoside-Binding Soluble 8 ELISA Kit, Po66 carbohydrate-binding protein ELISA Kit, Prostate carcinoma tumor antigen 1 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL8 in the samples is then determined by comparing the OD of the samples to the standard curve